Genetic engineering: Planning bugs

Designer bugs: structural engineering to build a better mouse model.

Many animal models of bacterial diseases are hampered by differences in tissue tropism and the course of pathogenesis. In a recent issue of Cell, by rationally mutating a surface invasion protein (InlA) to have higher binding affinity for its cognate host receptor (E-cadherin), Wollert et al. were able to "murinize"Listeria monocytogenes, creating a strain capable of invading intestinal epithel...

Strategic Technology Engineering Planning

This chapter presents a methodical strategic technology engineering planning (STEP) approach, to effectively cope with the design complexity in service-oriented architecture and manage the strategic planning of solution development of information systems. This holistic model comprises four modules: Want-Is-Target (WIT) model, Transition and Alignment Grid (TAG), Comprehensive Architecting Proce...

Engineering Route Planning Algorithms

Algorithms for route planning in transportation networks have recently undergone a rapid development, leading to methods that are up to three million times faster than Dijkstra’s algorithm. We give an overview of the techniques enabling this development and point out frontiers of ongoing research on more challenging variants of the problem that include dynamically changing networks, time-depend...

Genetic engineering.

Genetic engineering

BglII 5’...A_GATCT...3’ (5’overhang) BamHI 5’...G_GATCC...3’ (5’overhang); notice: MboI and Sau3AI = 5’..._GATC...3’ EcoRI 5’...G_AATTC...3’ (5’ overhang) HpaII 5’...C_CGG...3’ (5’ overhang); notice: CpG methylation blocked HindIII 5’...A_AGCTT...3’ (5’ overhang) KpnI 5’...GGTAC_C...3’ (3’ overhang) NcoI 5’...C_CATGG...3’ (5’ overhang); notice: Kozak sequence (RCC)ACCATGG NotI 5’...GC_GGCCGC......